Macrocyclic glycopeptides preparation

Because plasma and urine are both aqueous matrixes, reverse-phase or polar organic mode enantiomeric separations are usually preferred as these approaches usually requires less elaborate sample preparation. Protein-, cyclodextrin-, and macrocyclic glycopeptide-based chiral stationary phases are the most commonly employed CSPs in the reverse phase mode. Also reverse phase and polar organic mode are more compatible mobile phases for mass spectrometers using electrospray ionization. Normal phase enantiomeric separations require more sample preparation (usually with at least one evaporation-to-dryness step). Therefore, normal phase CSPs are only used when a satisfactory enantiomeric separation cannot be obtained in reverse phase or polar organic mode. [Pg.328]

The Preparation of the Pirkle Stationary Phases The Preparation of Cellulose and Amylose Stationary Phases The Preparation of the Macrocyclic Glycopeptides Phases The Preparation of the Cyclodextrin Based Stationary Phases Column Packing Techniques... [Pg.547]