Lytic enzyme system yeast cell

The lytic enzyme systems, active against yeast cell walls, usually contain l,3-/ -glucanases, proteases, mannanases, chitinases, and 1,6-) -glucanases. The proportion of those enzyme activities, their action pattern, synergism, and dependence on inhibitors constitute the activity profile... 

The experiment based on the two-level factorial design as described by Box and Wilson (19) was carried out in order to check the influence of the individual glucanases (g ) of Streptomyces sp. 1228 lytic enzymes system on the degree of yeast cell lysis (y). 

Microbial cell-wall-lytic enzymes are widely used in the laboratory for cell breakage, proto-plasting of yeasts and bacteria, and for studies of the structure and composition of microbial cell walls (J ). Recently lytic systems have come under consideration as a specific and chemically mild way to rupture microbial cells on an industrial scale (2 ). There appear to be attractive commercial applications of lytic systems for the recovery of enzymes, antigens and other recombinant products accumulated within cells, for upgrading of microbial biomass for food and feed uses (4 5) and for the manufacture of functional biopolymers from cell wall carbohydrates (6). 

Many microorganisms produce enzymes that lyse the cell wall of yeast. The most extensive work has been done with the lytic system from Arthrobacter sp., Cytophaga sp., Oerskovia, Bacillus circulans, Rhizopus, Tri-choderma, Penicillium, Pellicularia sp., Rhizoctonia, and Streptomyces sp. (3-9). 

Figure 8 shows a simulation of enzyme recovery from the wall, cytosol and mitochondria. The concentrations of recoverable enzyme are normalized to the initial amount of enzyme present in the cell site. The curves rise as enzyme is released from a site, then fall as it is hydrolyzed. It may be seen that the lytic system is usable even as a crude preparation to recover wall linked yeast enzymes in 60 to 80% yield. The yield of yeast wall enzyme depends on... 

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