Lytechinus pictus

Anthocidaris sp., 151 Arbaciapunctata, 455 Hemicentrotus sp., 151 Lytechinus pictus, 860 Strongylocentrotus purpuratus, 860, 880 Starfish, Asterias rubens, 656... 

Unfertilized eggs of S. purpuratus can also be dejellied by brief exposure to a buffer containing 27 mM KCl, 512 mM NaCl, 2 mM HEPES, pH 8.0, and 1 mM EDTA (Zhang and Ruderman, 1993). Lytechinus pictus eggs can also be dejellied in a similar buffer containing 0.1 mM EDTA (Zhang and Ruderman, 1993). 

Figure 7.5 2D 1H-31P -HMQC-T0CSY of membrane vesicle fraction of nuclear envelope of Lytechinus pictus as explained in text. The projection of the 2D peaks onto the left side of the figure gives a NMR spectrum. Along the top, the projection gives the NMR spectrum. [Reproduced from Larijani et ai. (2000) Lipids 35(11) 1289-1297]...

A few data are also available on the mt DNA in eggs of the sea urchin species Lytechinus pictus and Strongylocentrotus purpuratus. 

Grunstein, M., and Schedl, P., 1976, Isolation and sequence analysis of sea urchin (Lytechinus pictus) histone H4 messenger RNA, J. Mol. Biol. 104 323. 

Shimomura, H., Suzuki, N., and Garbers, D.L. (1986) Derivatives of speract are associated with the eggs of Lytechinus pictus sea urchins. Peptides, 7, 491-495. 

Fig. 2. Pattern of incorporation of C-labeled amino acids by artificially activated (butyric acid) nonnucleate fragments and whole eggs of Lytechinus pictus, in six sets of experiments, 10 amino acids tested in duplicate in each 1...

Fig. 3. Valine- C incorporated into protein in 1 hour by eggs of Lytechinus pictus starting at the indicated times, (a) and (b) Whole eg and nonnucleate fragments of the same experiment, (c and d) Two separate experiments. Incubations at 20°C in 0.3 ml of valine- C (specific activity 200 C/mole) in seawater at 0.4 jttC/ml and at egg concentrations near 1% (3600 eggs) by volume. Activation with butyric acid. Vertical lines show spread of duplicates. From Tyler (1966).

Fig. 5. Action of puromycin on incorporation of valine- " C into protein by unfertilized and fertilized eggs of Lytechinus pictus. The eggs were incubated with the valine- C for 60 minutes at 20°C, starting 30 minutes after addition of the puromycin, which occurred at 30 minutes after fertilization for the fertilized eggs. Each tube contained in 0.3 ml final volume, 10,000 luifertilized or 2000 fertilized eggs, puromycin at the indicated concentrations, and 0.25 iC valine- C (specific activity 208.5 / C// mole). Each set of experiments was run in duplicate and the error bars are drawn between the two values. From Tyler et al. (1968).

PiATiGORSKY, J., OzAKi, H., and Tyler, A. (1967). RNA- and protein-synthesizing capacity of isolated oocytes of the sea urchin Lytechinus pictus. Develop. Biol. 15, 1-22. 

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