Lysozyme-urea separation

An example of the application of RARE for rapid image acquisition is shown in Fig. 19, in which a single frame is shown from a series of 2-D images acquired of an oscillatory chemical reaction occurring within a fixed bed. Relaxation contrast is used to discriminate between the reaction products Mn " and Mn (49). In this example, MR offered the opportunity to map the detailed structure of the fixed bed and the product distribution within it. This pulse sequence has also recently been applied to obtain quantitative images of the evolution of a lysozyme urea separation within a chromatography column (50). 

Tryptic Digestion. The ozonized lysozyme solution after treatment with dithiothreitol in 9 M urea at pH 8.0 as described above, was carboxymethylated with sodium iodoacetate (0.02 M) at pH 4.6 and 25°C. for 24 hr, and purified by passage through a Sephadex G-25 column. Hydrolysis of lysozyme by trypsin (1%), and separation of the products were performed under the conditions as previously described by Jolles (12). 

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