Lysozyme spin labeled study

Other ESR and NMR studies have also been dealt with in Section VI. Gallo et al. (1971) and Teichberg et al. (1974) used ESR to explore the binding of metals to lysozyme. In addition, Berliner et al. (1983) used NMR as well as ESR to study the Mn(II) binding site of a-lactalbumin. More recently, Musci et al. (1987) used ESR and NMR to determine certain intramolecular distances between spin-labeled Met-90 and the metal binding site, as well as certain resolvable protons. 

Zhang ZW, Fleissner MR, Tipikin DS, Liang ZC, Moscicki JK, Earle KA, Hubbell WL, Freed JH (2010) Multifrequency electron spin resonance study of the dynamics of spin labeled T4 lysozyme. J Phys Qiem B 114 5503-5521... 

Neri et alF describe the used of the water-soluble nitroxide spin label, TEMPOL, to map the solvent-exposed surface of hen egg-white lysozyme. Pintacuda and Otting have used the Gd chelate, Gd(DTPA-MBA), for the same purpose in studies of ubiquitin. The latter group reports that the correlation of maps generated using TEMPOL and the Gd agent were poor. They recommend the use of the latter, which is more strongly relaxing and less hydrophobic, hence less likely to bind to specific hydrophobic patches of the protein surface. 

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