Lysozyme messenger

Salser, W., Gesteland, R.F., Ricard, B. Characterization of lysozyme messenger and lysozyme synthesized in vitro. Cold Spr. Harb. Symp. quant. Biol. 34, 771-780 (1969)... 

ScHWEiGER et al., 1972). And although lysozyme messenger shows almost no decay at all, in vivo lysozyme synthesis stops. Both, translational control of available messenger RNA and a differential change in the messenger decay rates seem to be involved. This control seems different from that responsible for the mechanism of shut-off of host translation. 

RNA dependent enzyme synthesis in the DEAE system is linear for 7-8 minutes. The half life of lysozyme messenger RNA appears to be approximately 7 minutes. A similar stability was measured for total RNA (unpublished data). This half life is longer than the one in vivo (Adesnik and Levin-THAL, 1970 ScHWEiGER et aL, 1972). The in vitro system may lack nucleases (DEAE cellulose chromatography of the Si 00 protein) or part of the natural mRNA degrading enzymes (Wetekam and Ehring, 1973)-... 

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