Lysosensor probes

LysoTracker and LysoSensor probes Several commercially available weakly basic amines can be used to stain lysosomes. They selectively accumulate in acidic organelles when applied in very low concentrations (50 nM) and directly before imaging. For live cell imaging, keep in mind that lysosomal probes can exhibit an alkalinizing effect on the lysosomes, such that longer incubation time can induce an increase in lysosomal pH (129). 

Also pH-imaging has been described with FLIM using chemical dyes that display a pH-dependent shift in hfetimes such as carboxy-SNAFL-1 and -SNAFL-2 [114,116], [117,118], BCECF [119, 120], carboxyfluorescein [121] and the novel low-PH LysoSensor probes [ 122], for a comparison see also [ 123]. 

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