Lysine cytochrome

Second-order rate constants (25°C) for the oxidation of singly modified CDNP-Lysine Cytochrome c Derivatives by Co(phen)3 + (kCo), Fe(CN)63- (kFe) and PCu(II) (kCu) at... 

Reaction with Chemically Modified Cytochrome c. Chemically modified (CDNP) cytochrome c derivatives have been prepared by Margoliash and colleagues (22). Lysine residues react as in (17),... 

Figure 10. View of cytochrome c with positions of exposed heme edge (block) and lysine modifications (sequence numbers for a-carbons) shown. The smaller circles indicate the relative effectiveness of modifications on rate constants for the reaction of Cyt c(II) + PCu(II) ( see Table III). Preferential interaction with PCu(II) in the direction 25,27,13,87 is indicated.

In one series of experiments the cytochrome c oxidase mutations replaced acidic residues by neutral ones, and some of them were thus expected to alter the nature of binding of the protein to cytochrome c. From the pattern of dependence of the heme c to Cua electron-transfer rate constant on these mutations it was deduced that the binding of cytochrome c to cytochrome c oxidase is mediated by electrostatic interactions between four specific acidic residues on cytochrome c oxidase and lysines on cytochrome c. In another series of experiments, tryptophan 143 of cytochrome c oxidase was mutated to Phe or Ala. These mutations had an insignificant effect on the binding of the two proteins, but they dramatically reduced the rate constant for electron transfer from heme c to Cua- It was concluded that electron transfer from... 

Cleavage of the oxirane C-0 bond produces a zwitterionic intermediate (Fig. 10.22), which that can undergo chloride shift (Pathway a) to 2,2-dich-loroacetyl chloride (10.90) followed by hydrolysis to 2,2-dichloroacetic acid (10.91). Furthermore, the zwitterionic intermediate reacts with H20 or H30+ (Pathway b) by pH-independent or a H30+-dependent hydrolysis, respectively. The pH-independent pathway only is shown in Fig. 10.22, Pathway b, but the mechanism of the H30+-dependent hydrolysis is comparable. Hydration and loss of Cl, thus, leads to glyoxylyl chloride (10.92), a reactive acyl chloride that is detoxified by H20 to glyoxylic acid (10.93), breaks down to formic acid and carbon monoxide, or reacts with lysine residues to form adducts with proteins and cytochrome P450 [157], There is also evidence for reaction with phosphatidylethanolamine in the membrane. 

H. Cai, F. P. Guengerich, Acylation of Protein Lysines by Trichloroethylene Oxide , Chem. Res. Toxicol. 2000,13, 327 - 335 H. Cai, F. P. Guengerich, Reaction of Trichloroethylene and Trichloroethylene Oxide with Cytochrome P450 Enzymes Inactivation and Sites of Modification , Chem. Res. Toxicol. 2001, 14, 451 - 458. 

Fig. 3. Schematic of cytochrome c, indicating the positions of invariant charged lysines with respect to the heme reactive center...

Cardiolipin forms also tight complexes, with the adenine nucleotide translocator (ATM) affecting its translocator activity (Beyer and Nuscher, 1996). Six cardiohpin residues are tightly bound to lysines (Beyer and Klingenberg, 1985). Removal of these lipids renders the translocator inactive, but activity can be reconstituted by adding cardiolipin. It has also a pivotal role as a boundary hpid of various proteins such as NADH ubiquinone oxireductase (Hoch, 1992) or cytochrome c oxidase (Ushmorov et al, 1999 Vik et al, 1981). 

Enzymatic hydroxylation of biological molecules is often catalyzed by hydroxylases. These types of enzymes are either oxygenases or peroxidases, in which the source of oxygen is O2 or H2O2, respectively. Cytochrome P-450-dependent enzymes represent a common class of enzymes that carry out hydroxylation reactions. L-Carnitine is a metabolite isolated from many organisms and its biosynthesis begins with the enzymatic hydroxylation of trimethyllysine. The intermediate, 3-hydroxyl-e-(A(A(ALtrimethyl)-L-lysine, is further... 

The following is review on the molecular and physical properties of this class of monooxygenases, which are also known as hydroxylases. A typical monooxygenase reaction is the hydroxylation of an alkane to an alcohol which involves a reduced cosubstrate that reduces a second atom within the O2 molecule to form water. Flavin-containing monooxygenases include lysine oxygenase and 4-hydroxybenzoate hydroxylase. Reduced pteri-dines are involved in the phenylalanine hydroxylase and tryptophan hydroxylase reactions. See also Cytochrome P-450... 

Nonspecific cytochrome P450-mediated oxidation involves enzyme-catalyzed formation of reactive oxygen species (superoxide anions and hydroxyl radicals), which oxidize susceptible amino acids such as proline, arginine, lysine, and histidine. 

Planar chirality is a valuable feature of the ferrocene chemistry (Chart 2B,C) (205). This unnatural chirality type has attracted attention of several groups. Sadeghi and co-workers have demonstrated that the planar chiral ferrocenes are recognized by cytochrome c peroxidase (206). The rate constants for the oxidation of R and S enantiomers by the wild type enzyme equal 2.9 x 106 and 1.6 x 106 M 1 s respectively. Interestingly, the enantioselectivity inverts for the aspartate 34 for lysine mutant and the rate constants become equal to 5.9 x 106 and 14.8 x 106 M-1 s-1, respectively. The discrimination of planar chiral ferrocenes is the case, but the stereoselectivity factors are lower than 3. 

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