Location of post-translational modifications and prosthetic groups

11 Location of post-translational modifications and prosthetic groups 

Again in the first step of the reaction between 1,3-dihydroxyacetone monophosphate (5.41) and glyceraldehyde-3-phosphate catalysed by aldolase to form fructose-1,6-diphosphate or the reverse reaction, a ketimine (5.42) is formed between the substrate and the e-amino group of a Lys residue in the enzyme. The formation of this intermediate (5.42) can be demonstrated similarly by trapping it as a secondary amine using NaBH4 to reduce the ketimine. The glyceraldehyde-3- 

CCTTTAGTACTTTCGGCCGTC... T Sequence of DNA synthesized on primer GGAAATCATGAAAGCCGGCAG...5 Sequence of template, single-stranded DNA GlyAsnHisGluSerArgGln Amino-acid sequence 

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