Liver glucose-6-phosphatase

An increase in liver glucose-6-phosphatase like that in scurvy also occurs in starvation and may be produced by the inanition of scurvy along with the marked reduction of liver glycogen and reduced glucose tolerance commonly seen (M28, S24, B6). Inanition has not been observed to cause some of the other enzyme changes. ATPase, for example, increases in heart and skeletal muscle of fasted guinea pigs (MIO) in contrast to the fall observed in the scorbutic animals (S22). 

G. Weber, C. Allard, G. de Lamirande and A. Cantero, Increased Liver Glucose-6-Phosphatase Activity after Cortisone Administration, Biochim. biophys. Acta 16, 618-619 (1955). 

Relation of Liver Glucose-6-phosphatase to Glycogen Formation ... 

Fio. 3. pH Activity curve of liver glucose-6-phosphatase from fed and fasted, normal and diabetic rats. Citrate buffer (0.1 M) was used for the pH range 4.7-6.S, and 0.1 M borate buffer for pH 7.6-9.0. Each curve represents the mean of six or more animals. Reprinted from Ashmore el al. (1954). 

L. Hue and H,G. Hers recently described a method employing double labelled glucose (2 - and U-C ) to assay for the activity of liver glucose-6-phosphatase in vivo. The test is based on the principle that, a differential in the label of as compared to glucose is produced during the recycling of glucose at the level of the phosphohexose-isomerase reaction. 

Segal, H. L., and Washko, M. E., 1959, Studies of liver glucose-6-phosphatase. III. Solubilization and properties of the enzyme from normal and diabetic rats, /. Biol. Chem. 234 1937. 

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