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Liquid microcarrier system

By utilizing fluorocarbon fluids containing pentafluorobenzoyl chloride, a liquid microcarrier system has been developed capable of use with a variety of cell types including normal human fibroblast. In this configuration, cells on the surface of a coarse oil dispersion ( 150 /im diameter) exhibit exponential growth (Figure 7). In addition, a microcarrier based on silicone oil has been formed and used to culture mouse fibroblasts (12-13). [Pg.595]

B.9.1.6 Mixing Time in Three-Phase (Gas-Liquid-Solid) System There are no reported experimental data on gas-liquid-solid systems that are relevant to animal cell culture using microcarrier beads as support for the cells. As mentioned earlier in the case of gas-liquid and solid-liquid systems, because of (i) the low density difference (Ap 30-50 kg/m ) and (ii) low aeration rates, there is insignificant impact of introduction of the solid and gas phases. Further, if an upflow impeller is used, the power drop due to aeration is less than 10% at low aeration rates (Fig. 7A.6). Therefore, Equation 7B.11 can be used in this case also. [Pg.271]


See other pages where Liquid microcarrier system is mentioned: [Pg.601]    [Pg.601]    [Pg.279]    [Pg.279]    [Pg.280]    [Pg.259]    [Pg.119]    [Pg.229]    [Pg.270]    [Pg.271]    [Pg.766]   


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Microcarriers

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