Liquid chromatography/Fourier transform structure elucidation

Table 5.7 Theoretically predicted polypeptides from the trypsin digestion of S-lacto-globulin (/3LG) . Reprinted from J. Chromatogr., A, 763, Turula, V. E., Bishop, R. T., Ricker, R. D. and de Haseth, J. A., Complete structure elucidation of a globular protein by particle beam liquid chromatography-Fourier transform infrared spectrometry and electrospray liquid chromatography-mass spectrometry - Sequence and conformation of /3-lactoglobulin , 91-103, Copyright (1997), with permission from Elsevier Science...

The analysis of lipids is dominated by chromatographic methods such as gas chromatography (GC) and high-pressure liquid chromatography (HPLC) and some other physical methods such as thermo analysis. Nuclear magnetic resonance (NMR) spectroscopy covers only a small part of lipid analysis. In most cases it is used for qualitative analysis such as structure elucidation of lipids. Unlike the other methods NMR spectroscopy is coupled with computer technology, and its development is as fast as that of computer software and hardware. In 1991 the Nobel Prize for chemistry was given to Professor Ernst for the development of Fourier Transform NMR spectroscopy (FT-NMR). The capabilities of these methods even for the elucidation of the three-dimensional structures of small molecules up to macromolecules are impressive. 

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