Liposomes cryoprotectants used

If the liposome preparations are intended to be stored for longer time periods, they may be frozen or lyophilized, provided that they are prepared in a phosphate buffer that contains a cryoprotectant. We use an iso-osmolar phosphate-mannitol buffer of the following composition 20 mM phosphate buffer (0.53 g/L KH PO plus 2.87 g/L Na2HP0 2Hp) plus 230 mM mannitol (42.0 g/L mannitol), (PB-Man)... 

Because water is an essential component of the liposomal bUayer structure, freezing can promote damage due to dehydration. Use of cryoprotectants and control of the freezing rate that can minimize the formation of large ice ciystals are of utmost importance. To achieve stability, the quality of phospholipids and the production process must be reproducible. 

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