Lipoprotein subunits separation

Several studies have shown (cf. review by Bakerman and Wasem-iller, 1967 Engelman et al., 1967 Terry et al., 1967 Engelman and Morowitz, 1968a,b) that membrane dissociation could be limited to the separation of lipoprotein subunits by the use of appropriate agents and conditions. On the other hand, the use of selected solvents has permitted the isolation and study of structural protein. Data from such investigations made with erythrocyte membranes constitute the most complete set available (Bakerman and Wasem-iller, 1967 Zahler, 1968) and for this reason will be used in the following analysis of membrane dissociation mechanism. 

The method has been used for the separation of enzymes, hormones, RNA-DNA hybrids, ribosomal subunits, subcellular organelles, for the analysis of distribution of samples of polysomes and for lipoprotein fractionation. 

Separations RNA-DNA hybrids, ribosomal subunits etc. DNA. plasma lipoproteins, lysosomes, mitochondria, peroxisomes etc. 

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