Lipoprotein subunits dissociation

Several studies have shown (cf. review by Bakerman and Wasem-iller, 1967 Engelman et al., 1967 Terry et al., 1967 Engelman and Morowitz, 1968a,b) that membrane dissociation could be limited to the separation of lipoprotein subunits by the use of appropriate agents and conditions. On the other hand, the use of selected solvents has permitted the isolation and study of structural protein. Data from such investigations made with erythrocyte membranes constitute the most complete set available (Bakerman and Wasem-iller, 1967 Zahler, 1968) and for this reason will be used in the following analysis of membrane dissociation mechanism. 

Concentrated acetic acid (Table IV, 7) probably induced considerable dissociation of lipoprotein subunit components. This solvent falls in the group used by Zahler (1968) (Table IV, 6) for this very purpose. 

There are some oligomeric proteins—for instance a-crystallin [47] and high-density lipoprotein [48J—which did not dissociate into subunits, though 90% of lysyl residues were succinylated. Succinylation of aldolase [40] and ceruloplasmin [43] in neutral solution led to intermediary subunits only, whereas the final dissociation into the... 

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