Lipids extraction from hairless mouse

Lipid Extracts. Lipids were extracted from the hairless mouse stratum corneum using a modified method based on methods developed by Bligh and Dyer (37) and Elias and coworkers (17.20.21). 

Differential Scanning Calorimetry (DSC) Studies. Hairless mouse abdomen stratum corneum, extracted lipids and protein residues were studied with a Perkin Elmer 4 differential scanning calorimeter (DSC) equipped with a thermal analysis data system (TADS). Scanning rates were 10°C per minute over the temperature region -10 to 237°C. Stratum corneum, extracted lipid and protein residue samples obtained from the abdomen of the hairless mice (average 10 mg/sample) were studied in the desiccated state following evaporation of any residue water or solvents by vacuum drying at 10 4 Torr. 

Figure 2. DSC thermograms for hairless mouse stratum comeum, extracted lipids and protein residue from -10 to 237°C.

Figure 3. Transmission FTIR spectra of desiccated, hairless mouse stratum corneum, protein residue and extracted lipids from 4000 to 2400 cm-. ...

Figure 11. Wavenumber versus temperature plot for desiccated, hairless mouse extracted lipids in the CH2 asymmetric C-H stretching region from 2916 to 2925 cm"l during the heating and cooling cycle.

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