Starch Lintner soluble

Special Starch Use starch designated as Starch (Lintner) Soluble (Baker Analyzed Reagent, Catalog No. 4010). Before using new batches, test them in parallel with previous lots known to be satisfactory. Variations of more than 3° diastatic power in the averages of a series of parallel tests indicate an unsuitable batch. 

Lintner degree. Amount of reduced compounds calculated as maltose that is produced by 25 g of malt from soluble starch in 30 min at 20°C. 

The characteristic of beta amylases of reaching a limit in the hydrolysis of starches gives the investigator a simple criterion for ascertaining whether his amylase preparation has been freed from alpha amylase, which usually accompanies beta amylase in nature. If the hydrolysis of Lintner s soluble potato starch at pH 4.5 ceases when approximately 64%... 

The ratio of dextrinogenic to saccharogenic activities for pancreatic amylase, measured with Lintner s soluble potato starch under comparable conditions at 40°, is approximately 2 to 45,57 The achroic point is reached in the hydrolysis of potato starch by highly purified pancreatic amylase when approximately 20% of the glucose linkages of the starch have been broken.41... 

Influence of Dialysis During Hydrolysis upon the Extent of the Hydrolysis of Lintner s Soluble Potato Starch by Pancreatic Amylase (Data of Alfin and Caldwell 1)... 

The amylase of Aspergillus oryzae causes a very rapid decrease in the viscosity of its substrates and a very rapid disappearance from its reaction mixtures of products which give color with iodine. When examined under favorable conditions71 at 40° with Lintner s soluble potato starch, the achroic point was reached with highly purified maltase-free amylase when approximately 12% of the glucose linkages of the substrate had been ruptured. 

When measured at 40° with Lintner s soluble potato starch, the ratio of the dextrinogenic to the saccharogenic activities is approximately 6 to 1 for the amylase of Aspergillus oryzae. This value is given by both crude and purified preparations of the amylase if the measurements are carried out under comparable conditions. This constancy in the ratio of these two activities has led to the conclusion that, like pancreatic amylase, the amylase of Aspergillus oryzae is not accompanied in nature by beta amylase. Reacting mixtures of the amylase of Aspergillus oryzae and starch exhibit alpha mutarotation.72... 

Similarly, the addition of maltose to reaction mixtures failed to influence the extent of the hydrolysis of Lintner s soluble potato starch... 

Very highly purified preparations of alpha amylase of malted barley give a value of approximately 4 to 1 for the ratio of their dextrinogenic to their saccharogenic activities when the measurements are made at 40° with Lintner s soluble potato starch.81 Under the same conditions approximately the same value is obtained with products precipitated by alcohol from malted barley extracts which had been treated to inactivate beta amylase.23 81 However, a constant value for these ratios is not proof that beta amylase is entirely absent. There is at present no satisfactory way of making certain that malted barley alpha amylase is not contaminated with traces of beta amylase. The crystallization of alpha amylase from malted barley has been reported since this manuscript was written.79... 

Robin J.R, Mercier C., Charbonniere R., Guilbot A., Lintnerized starches. Gel filtration and enzymatic studies of soluble residues from prolonged acid treatment if potato starch. Cereal Chem., 51,1974, 389-406. 

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