Linker design

This loading approach is successful for the relatively unhindered 6-hydroxyl, but proved to be inefficient for more sterically encumbered alcohols. A modification of the abovementioned method has been developed to enable attachments through these more hindered sites.9 This process is accomplished with inexpensive materials, and the linker is compatible with a variety of reaction conditions. Moreover, it allows for facile recycling of the polymer support for further use once the final carbohydrate assemblage is cleaved from the resin. 

4 SOLID SUPPORT GLYCAL ASSEMBLY VIA 1,2-ANHYDROSUGAR DONORS 

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Jahnke, W., et al., Second-site NMR screening and linker design. CurrTop Med Chem, 2003, 3, 69-80. 

In many cases, the aforementioned inherent properties of natural hnkers of mnltidomain proteins have served as a basic guide to linker design for the constraction of man-made fusion enzymes. For the engineering of reeombinant fusion proteins, three main types of linkers are often employed these include flexible linkers, rigid linkers, and linkers that can be eleaved, either proteolytically orehemically [185],... 

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