Linear salt gradient

Figure 1. Separation of Ct from CL and p-glucosidase components of T. koningii cellulose on DEAE-Sephaaex. Protein 1 eluted under starting conditions proteins 2 ana 3 with linear salt gradient (26).

An extensive comparison between these two gradient modes in lEX-RP separation of cytosolic proteins from Escherichia coli revealed a more effective fractionation for a linear salt gradient [70]. Fewer major proteins were distributed to multiple second-dimension fractions. 

Figure 2, Typical Chromatographic Separation of Proteins from an Ion Exchange Support using a Linear Salt Gradient. In this chromatogram, the first two peaks (I and 2) are IgG, the next peak (3) is albumin, and the final peak (4) is fibrinogen. The physiological salt concentration was surpassed in the linear gradient around 10 minutes, between the elution of IgG and albumin. Separation conditions included elution at 37 C. This figure was reprintedfrom Journal of Chromatography, Vol,...

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