Ligninolytic high-redox potential laccases

The first successful example of the directed evolution of fungal laccase involved the laccase from the thermophile ascomycete Myceliophihora thermophila laccase (MtL). This study led to subsequent directed evolution experiments in S. cerevisiae with several high-redox potential ligninolytic oxidoreductases (see below). MtL was subjected to 10 cycles of directed evolution to enhance its functional expression in S. cerevisiae [38]. The best performing variant of this process (the T2 mutant that harbored 14 mutations) exhibited a total improvement of 170-fold in activity its expression levels were enhanced 8-fold and the around 22-fold. The... 

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