Stopped-flow light scattering

Initial Rapid Processes in Retrogradation of Amylose Observed by the Light-Scattering, Stopped-Flow Method... 

Figure I. Schematic of the light-scattering stopped-flow apparatus. Samples (alkaline amylose arid acetic acid solutions) are placed in the reservoirs A and B. M, monochromater ( = 320 nm) F, a cut-off filter with half-transmittance wavelength at 295 nm PM, photomultiplier PM was set at 90° to the incident beam.

Milon, A., T. Lazrak, A. M. Albrecht et al. 1986. Osmotic swelling of unilamellar vesicles by the stopped-flow light scattering method. Influence of vesicle size, solute, temperature, cholesterol and three a, Q)-dihydroxycarotenoids. Biochim. Biophys. Acta 859 1-9. 

Stop-flow experiments have been performed in order to study the kinetics of micellization, as illustrated by the work of Tuzar and coworkers on PS-PB diblocks and the parent PS-PB-PS triblocks [63]. In these experiments, the block copolymers are initially dissolved as unimers in a nonselective mixed solvent. The composition of the mixed solvent is then changed in order to trigger micellization, and the scattered light intensity is recorded as a function of time. The experiment is repeated in the reverse order, i.e., starting from the block copolymer micelles that are then disassembled by a change in the mixed solvent composition. The analysis of the experimental results revealed two distinct processes assigned as unimer-micelle equilibration at constant micelle concentration (fast process) and association-dissociation equilibration, accompanied by changes in micellar concentration (slow process). 

Bemocco, S., Ferri, F., Profumo, A., Cuniberti, C., and Rocco, M. (2000). Polymerization of rod-like macromolecular monomers studied by stopped-flow, multiangle light scattering Set-up, data processing, and application to fibrin formation. Biophys.J. 79, 561—583. 

Figure 3. View of the whole apparatus. Right, a Union Giken RA-1300 stopped-flow spectrophotometer, gas-pressure-driven type) in light-scattering mode left, a Union Giken RA-450 rapid data processor with a cathode ray monitor on the processer. The recorder is on the upper center shelf.

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