Light microscopy interfacing microscope with

A PC interfaced with a microscope photometer provides a powerful analytical tool working down to cellular and subcellular levels (with polarized light). With the relatively low cost of modem PCs and the wide availability of surplus components for light microscopy, the methodology is available to anyone who can combine microscopy with computer... 

Confocal microscopy (CM) is another microscope technique for apparent optical sectioning, achieved by exclusion of out-of-focus emitted light with a set of image plane pinholes. CM has the clear advantage in versatility its method of optical sectioning works at any plane of the sample, not just at an interface between substances having dissimilar refractive indices. However, other differences exist which, in some special applications, can favor the use of TIRF ... 

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