Light microscopy autoradiography

Teal, J.J., and Holtzman, S.G. Discriminative stimulus effects of cyclazocine in the rat. J. Pharmacol Fxp Ther 212 368-376, 1980. Tempel, A. Gardner, E-L. and Zukin, R.S. Visualization of opiate receptor upregulation by light microscopy autoradiography. Proc Natl Acad Sci USA 81 3893-3897, 1984. 

Barnes PJ, Basbaum CB, Nadel JA, Roberts JM. Localization of 3-adrenoreceptors in mammalian lung by light microscopy autoradiography. Nature 1982 299 444-447. 

Jenkins EC. Wire-loop application of liquid emulsion to slides for autoradiography in light microscopy. Stain Technol 1972 47 23-26. 

Different light microscopy techniques, such as autoradiography and quantitative fluorescence microscopy (QFM), have been used to examine the influence of penetration enhancers on percutaneous absorption. 

The extent of lumen penetration into earlywood, latewood and wood rays is preferably determined by examination of the cross section of the bondline, and many techniques have been successfully used for this purpose light microscopy [29, 30], transmitted and reflected microscopy [31-33], (epi-)fluorescence microscopy [2, 10, 26, 31, 34-40], fluorescence confocal laser scanning microscopy (CLSM) [34, 41 5], scanning electron microscopy (SEM) [32, 46 8], transmission electron microscopy (TEM) [49-52], SEM in combination with an energy-dispersive analyzer for X-rays (SEM/EDAX) [43, 53], X-ray microscopy [54], autoradiography [11] or combination of different microscopy techniques [55]. Kamke... 

The results will rely on the sampling method (individual organ or whole-body study) and on the techniques used (paraffin embedding, cryomicrotomy, light or electron microscopy, autoradiography...)... 

Autoradiography is a technique for locating radioactive compounds within cells it can be conducted with light or electron microscopy. Living cells are first exposed to the radioactive precursor of some intracellular component. The labeled precursor is a compound with one or more hydrogen ( H) atoms replaced by the radioisotope tritium (3H) e.g., [3H]thymidine is a labeled precursor of DNA, and [3H]uridine is a labeled precursor of RNA (Chap. 7). Various tritiated amino acids are also available. The labeled precursors enter the cells and are incorporated into the appropriate macromolecules. The cells are then fixed, and the samples are embedded in a resin or wax and then sectioned into thin slices. 

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