Ligand in affinity chromatography

Entrapment, of ligand in affinity chromatography, 6 397 Entrepreneurial Technology... 

Due to the central role of the ligand in affinity chromatography, some factors should be considered when selecting a ligand for the purification of a protein ... 

Reactive dyes are common ligands in affinity chromatography for years.175 They have been used for the separation of a number of proteins, the most common being albumin176 and dehydrogenases.177... 

All osidic structures which can interact with proteins implicated in biological responses or phenomenons are, a priori, candidates as ligands in affinity chromatography. Here, we give some examples of oses... 

Kopperschlager, G., Bohme, H.-L, Hofman, E. (1982) Cibacron blue F3G-A and related dyes as ligands in affinity chromatography. In Advances in Biochemical Engineering (Fichter, A., editor), 25, 101-138, Berlin Springer-Verlag... 

The elution of [60]- and [70]fullerenes was measured in water-methanol as a function of temperature on a poly(octadecylsiloxane) phase.67 The retention was shown to be dependent on the surface tension of the stationary phase through a simple geometrical model in which the solute formed a cavity in the stationary phase. In affinity chromatography, it was demonstrated that low ligand density may be a requirement for specificity of binding.68... 

The principles of enzyme immobilization on solid phases and the requirements for such solid phases are in general the same as those applied for sorbents and protein ligands used in affinity chromatography. Some time ago the main requirements for the ideal matrix were formulated as follows [84] ... 

There are many types of affinity ligands that can be used in affinity chromatography. The wide range of ligands available for affinity chromatography makes this method a valuable tool for... 

Table 13.3 summarizes various covalent immobilization methods that are used in affinity chromatography. Each of these methods involves at least two steps (1) an activation step, in which the support is converted to a form that can be chemically attached to the ligand and (2) a coupling step, in which the affinity ligand is attached to the activated support. With some techniques, a third step, in which remaining activated groups are removed, is also required. The methods listed in Table 13.3 can be performed either in-house or can be used in the form of preactivated supports available from commercial suppliers (see list in Table 13.2) [25,36]. 

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