Ligands basic binders

Monodentate Ligands - The Simple Type 2.2.1 Basic Binders... 

Fig. 8.6. Relationships of the various mathematical models used in immunoassays to the basic general equation as derived by Fernandez et al. (1983). Those used in El A are discussed in detail in Chapter 15. F is the ratio total activity/bound activity, h, and I the concentration of unlabeled and labeled ligand (undiluted), respectively, F the volume of undiluted labeled ligand added to the mixture and Fab the volume of undiluted binder added ab the concentration of binding sites (undiluted) and ab i, the concentration in the incubation mixture. F is the total volume of the incubation mixture. H, the mass of ligand in the incubation mixture. R is the bound/free ratio, h the concentration of unlabeled ligand in the incubation mixture, i the concentration of the standard or unknown, io the concentration of ligand contributed by the label, B bound label and Aobound label at zero standard concentration n is a constant.

The basic concept on which the standard equilibrium radioimmunoassay is predicated is a competition between untagged ligand P and tagged ligand P for binding sites Q on a specific binder. Such a system can be represented by the equations ... 

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