Ligand oligonucleotide

This spectroscopy has been used as a sensitive and effective analytical technique for the characterization of specific ligand-oligonucleotide duplex non-covalent complexes [138], due to its specificity, sensitivity and quickness as well as its advantage in determining binding stoichiometry. 

Conceptually the most simple syntheses of complex molecules involve the joining of structural units in which all functional groups and all asymmetric centres are preformed. This technique can usually only be applied to compounds in which these units are connected by —C—X— bonds rather than C—C. It is illustrated here by the standard syntheses of oligonucleotides, peptides, and polydentate macrocyclic ligands. 

Critical to the success of this procedure was the development of a suitable temporary ligand that is sufficiently inert to conditions of oligonucleotide deprotection (typically aqueous ammonia, 6h at 50° C) and can subsequently be replaced by chloride. The nucleobase derivative 1-cyclohexamethyl-thymine 41 was found to satisfy these criteria and can be converted to the trans-Pt-Cl species by treatment with dilute HC1 (pH 2.3, 40°C, 48 h). This last step restricts the range of possible oligonucleotide sequences to those that are not susceptible to depurination, but clearly establishes a prototype system for future development. 

Escude C, Sun J-S (2005) DNA Major Groove Binders Triple Helix-Forming Oligonucleotides, Triple Helix-Specific DNA Ligands and Cleaving Agents. 253 109-148 Evans SV, see Schuman B (2007) 272 217-258 Van der Eycken E, see Appukkuttan P (2006) 266 1-47... 

Oligonucleotide probes may be labeled with small fluorescent molecules for detection of hybridization by luminescence. Fluorescent probes are widely used in assay systems involving bio-specific interactions (Chapter 9). Receptors for ligands may be localized in tissues or cells by modification of the ligand with the appropriate fluorophore. Targeted molecules may be... 

Electrostatically-controlled pre-association interactions have an important effect on rates for [Pd(dien)Cl]+ reacting with thione-containing nucleosides, nucleotides and oligonucleotides, as is often the case for reactions between metal complexes and this type of biological ligand. Interaction between the charged complex and the polyanionic oligonucleotide surface leads to an increase in both enthalpy and entropy of activation in the DNA or model environment (252). 

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