Ligand binding receptor-based

Sippl W. Binding affinity prediction of novel estrogen receptor ligands using receptor-based 3-D QSAR methods. Bioorg Med Chem 2002 10 3741-55. 

D, M L Smythe, TI Oprea, C L Waller, S M Green and G R Marshall 1996. VALIDATE A New hod for the Receptor-Based Prediction of Binding iVffinities of Novel Ligands. Journal of the rican Chemical Society 118 3959-3969. 

We have also used a non-radiometric-binding approach based on fluorescence polarisation [29], where a fluorescent label is used in place of a radiolabel. As the fluorescently tagged oxytocin binds to the receptor, its rotational velocity is reduced and the polarisation of the fluorophore increases. The displacement of the ligand may be measured by a decrease in polarisation. 

Tahtaoui C, Guillier F, Klotz P, Galzi JL, Flibert M, Ilien B (2005) On the use of nonfluo-rescent dye labeled ligands in FRET-based receptor binding studies. J Med Chem 48 7847-7859... 

E. A. L. Biessen, F. Noorman, M. E. van Teijlingen, J. Kuiper, M. Barrett-Bergshoeff, M. K. Bijsterbosch, D. C. Rijken, and T. J. C. Van Berkel, Lysine-based cluster mannosides that inhibit ligand binding to the human mannose receptor at nanomolar concentration, J. Biol. Chem., 271 (1996) 28024—28030. 

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