Ligand binding, equilibrium measurement

Receptor occupancy refers to the ratio of receptors occupied by a ligand at equilibrium and the total number of receptors available, usually expressed as a percentage of the total number of receptors. Since it is often not possible to quantify the total number of receptors or the number of receptors occupied by a ligand, another parameter called binding potential is often used to measure receptor occupancy. Binding potential (BP) refers to the ratio of the maximum number of receptors and the equilibrium dissociation constant of the drug, so that % receptor occupancy equals to... 

The principle of equilibrium fluorescence measurements is identical to that of radiolabelled ligand binding experiments, i.e., the ligand (L) binds to its receptor (R) and the formation of the complex (RL) is... 

Selected entries from Methods in Enzymology [vol, page(s)] Ligand Binding Measurements By equilibrium gel penetration, 117, 342 by gel chromatography, 117, 346 by ultrafiltration,... 

Equilibrium measurement of ligand binding typically yields the moles of ligand bound per mole of macromolecule, v, which is given by... 

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