Ligand binding assay spectrometry

Continuous-flow Ligand Binding Assays Based on Mass Spectrometry... 

Irth H Continuous-flow systems For ligand binding and enzyme inhibition assays based on mass spectrometry, chapter 5. 

As binding assays provide a means to characterize the affinity of test compounds to defined targets, they play a very important, not to say an essential role in the drug discovery process. Next to the advantage of effective quantitation, the use of a marker, i.e. a labeled ligand - either with a radioisotope or a fluorophore - has, however, also serious immanent disadvantages. As the performance of mass spectrometry continues to improve, it appears therefore obvious to conduct... 

Powell, K.D. Ghaemmaghami, S. Wang, M.Z. Ma, L Oas, T.G. Fitzgerald, M.C. A general mass spectrometry-based assay for the quantitation of protein—ligand binding interactions in solution. 

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