Lifetime-based decomposition of spectra

A fluorescence spectrum may result from overlapping spectra of several fluorescent species (or several forms of a fluorescent species). If each of them is characterized by a single lifetime, it is possible to decompose the overall spectrum into its components. 

Decomposition of the fluorescence spectrum is possible in pulse fluorometry by analyzing the decay with a three-exponential function at each wavelength 

The procedure in phase-modulation fluorometry is more straightforward. The sine and cosine Fourier transforms of the d-pulse response are, according to Eqs (6.30) and (6.31), given by 

Decomposition in real time is possible by measuring I / and M as a function of wavelength at a single frequency and by calculating Px and Qx by means of Eqs (6.21) and (6.22) 

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Lifetime-based decomposition of spectra into components is simpler in phase fluorometry. 

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