Library shared-pool

Non-Parallel Synthesised Libraries (Shared-Pool/ Collected Collections )... 

The shared-pool suppliers, as seen in Table 2 below, are capable of providing 70,000-450,000+ compounds each from stock, generally as dry powder/dry film or as solutions in DMSO (from cherry-picked selections or pre-plated sets). The cost of these libraries is relatively low, allowing discovery scientists the opportunity to screen a large number of structurally and pharmacophorically diverse compounds easily and cheaply. By screening a relevant subset from the compounds offered, the user often can use the hits as the basis for structure queries in sourcing analogues for... 

Now CheshireManager becomes the Fa ade of Cheshire Runtime. As described earlier in this chapter, the Cheshire environment is not thread-safe therefore, managing the CheshireManager object becomes a challenge. There are two options one is to create only one instance of the CheshireManager within the JVM and let all requests share it. The other is to create a pool of CheshireManager that implements the RMI Remote interface—each runs in its own JVM. We have discussed the pros and cons of each approach. Here we take the first approach. However, we still implement it using an object pool for readers who are interested in the second approach. We use Apache Org s Object Pool Library to illustrate how it works. 

DNA-recorded library is another approach to encode small molecule libraries on DNA templates. Instead of preparing a template pool in which every library compound has already been encoded, in a DNA-recorded library initially all templates share an identical sequence, or even are simply one template. Usually, the chemical reactions on each template are encoded spatially such as the split-pool-split method i.e. the initial template pool is separated into several batches for discrete reactions with various building blocks. Before all the sub-pools are combined, an enzymatic tag is added to the template to record the synthesis. For every step, an additional tag is added. As a matter of fact, DNA-recorded library, similar to Harbury s DNA routing strategy discussed above, does not use DNA to template the reactants into proximity and to increase the effective molarity. 

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