Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Leucine, aminomutase

Leucine eiminomutase catalyzes isomerization of leucine and /8-leucine. As with methylmalonyl CoA mutase, the reaction involves cleavage of the Co-C [Pg.306]

Poston (1984) showed that, in isolated rat tissues, about 5% of the catabolic flux of leucine was by way of aminomutase action to yield /S-leucine, and then isobutyryl CoA, with the remainder provided by the more conventional a-transamination pathway leading to the formation of isovaleryl CoA. In patients suffering from vitamin B12 deficiency, there is an elevation of plasma /S-leucine, suggesting that the aminomutase may act to metabolize /S -leucine arising from intestinal bacteria, rather than as a pathway for leucine catabolism. [Pg.307]

Leucine 2,3-aminomutase interconverts the a and /3 forms of leucine. Similar mutases are known for other amino acids including lysine, tyrosine, and serine. The resolving power of HPLC is useful in separating and quantitating the a and /3 derivatives of these amino acids. [Pg.257]

The o-phthaldehyde derivatives of the amino acids were separated on a Waters Resolve CI8 column (3.9 mm x 150 mm). For separating a- and /3-leucine, a mobile phase containing 20% acetonitrile and 80% 0.1 Af potassium phosphate (pH 7.5) was used. The effluent was monitored by a fluorometer with the excitation and emission wavelengths set at 365 and 418 nm, respectively. [Pg.257]

Rat liver was homogenized in 0.2 M potassium phosphate buffer (pH 7.0) containing 0.8% Triton X-100. The supernate obtained by centrifuging the homogenate at 40 OOOg for 30 minutes at 0°C was used to assay leucine 2,3-aminomutase. [Pg.258]


Methylmalonyl CoA mutase, leucine aminomutase, and methionine synthase (Figure 45-14) are vitamin Bj2-dependent enzymes. Methylmalonyl CoA is formed as an intermediate in the catabolism of valine and by the carboxylation of propionyl CoA arising in the catabolism of isoleucine, cholesterol, and, rarely, fatty acids with an odd number of carbon atoms—or directly from propionate, a major product of microbial fer-... [Pg.492]

In mammals, there are only three vitamin B12 -dependent enzymes methionine synthetase, methylmalonyl CoA mutase, and leucine aminomutase. The enzymes use different coenzymes methionine synthetase uses methylcobal-amin, and cobalt undergoes oxidation during the reaction methylmalonyl CoA mutase and leucine aminomutase use adenosylcobalamin and catalyze the formation of a 5 -deoxyadenosyl radical as the catalytic intermediate. [Pg.304]

Leucine catabolism also occurs by a second, minor pathway. The first step in this pathway is catalyzed by leucine aminomutase (Poston, 1984). Leucine ami-nomutasc is one of the three vitamin Bjj-requiring enzymes in mammalian tissues. [Pg.430]

Vitamin Bu is unique among all the vitamins in that it is the largest and most complex and because it contains a metal ion. 1 hismetal ion is cobalt. Cobalt occurs in three oxidation states Co, Co, and Co. The medicinal forms of the vitamin are cyanocobalamin and hydnoxocobalamin. In cyanocobalamin, a molecule of Cyanide is complexed to the Co atom. Cyanocobalamin is readily converted in the body to the cofactor forms methylcobalamin and 5-deoxyadenosylcobalamin. Methylcobalamin contains cobalt in the Co slate, where it acts as a cofactor for methionine synthase. 5 Deoxyadenosylcobalamin, which contains cobalt in the Co state, is the cofactor for methylmalonyl-CoA mutase. Vitamin Btj is also a cofactor for leucine aminomutase, anen7yme used in leucine metabolism (Poston, 1984). This enzyme appears not to have a vital function in metabolism. No more... [Pg.516]

CoA mutase, leucine aminomutase and methionine synthetase. Methionine synthetase is discussed in section 11.11.3.2. [Pg.382]


See other pages where Leucine, aminomutase is mentioned: [Pg.306]    [Pg.306]    [Pg.992]    [Pg.306]   
See also in sourсe #XX -- [ Pg.492 ]

See also in sourсe #XX -- [ Pg.516 ]




SEARCH



Aminomutase

Aminomutases

L-Leucine 2,3-aminomutase

Leucine, aminomutase metabolism

Leucine, aminomutase transamination

Vitamin leucine aminomutase

© 2024 chempedia.info