Lesion bypass oligonucleotide

For a given lesion, error-free or error-prone synthesis by a bypass polymerase is often determined by in vitro translesion synthesis assays (Figure 22.21). The assay involves in vitro DNA synthesis by a purified bypass polymerase from an oligonucleotide template containing a site-specific lesion. A DNA primer labeled with 32P at its 5 end is annealed to the damaged template prior to assembling the assay reactions. Following the polymerase reaction, products are separated by electrophoresis... 

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