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Lead acetate dichlorofluorescein reagent

Lead tetraacetate-2,7-dichlorofluorescein (A) lead tetraacetate (saturated solution) in glacial acetic acid (B) 2,7-dichlorofluorescein in water (1%, m/v, or lower, down to 0.2%, if background interferes with detection) A and B are mixed 1 1 (v/v), then toluene (19 1, v/v) is added Plate is dipped in reagent for 10s, then heated at 100°C for 3 min All carbohydrates with vicinal diol groups, oxidized by lead tetraacetate, are detected fluorimetric scanning (excitation 313nm, emission 366 nm) 50-100pmol... [Pg.445]

See other pages where Lead acetate dichlorofluorescein reagent is mentioned: [Pg.325]    [Pg.326]    [Pg.327]    [Pg.328]    [Pg.665]    [Pg.172]    [Pg.172]    [Pg.173]    [Pg.1778]    [Pg.196]   
See also in sourсe #XX -- [ Pg.325 , Pg.326 , Pg.327 ]



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Lead acetate

Lead acetate dichlorofluorescein

Lead reagent

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