LC-SPR

One of the most exciting developments with SPR is the development of coupled analytical techniques with surface plasmon resonance. While many other coupled analytical techniques such as LC-MS and GC-MS are well practiced in routine work, little research has been done coupling SPR to other analytical tools until very recently. The most promising coupling technique continues to be SPR-mass spectrometry (MS) and LC-SPR-MS. LC-SPR and SPR-fluorescence (SPFS) have gained considerable attention in recent years. We briefly review these hyphenated techniques and discuss their prospects in future SPR measurements. 

Visser and coworkers combined LC, SPR, and MS together and immobilized cGMP molecules to an SPR chip to monitor the binding and dissociation of proteins from a human lysate by sequential elution steps and SPR [51]. The eluted proteins were thereafter identified by LC-MS/MS. The data indicate that SPR-based chemical proteomics is a viable alternative for quantitative extraction and identification of small-molecule-binding proteins from complex matrices. In fact, SPR-MS technological innovations have grown considerably in the last few years and have even sparked enough interest that leads to a book chapter devoted to the topic [52]. 

Marchesini GR, Buijs J, Haasnoot W, Hooijerink D, Jansson O, Nielen MWF (2008) Nanoscale affinity chip interface for coupling inhibition SPR inununosensor screening with nano-LC TOF MS. Anal Chem 80 1159-1168... 

Compound quality is addressed with a rapid LC/UV/MS assay [12]. Many groups use compounds that were synthesized several years before or obtained from an outside source. Structure assignment inaccuracies, impurities, and degradants can cause SAR and SPR confusion for the drug-discovery team. 

Li and Jandik (1991) (C4 i4) benzenesulfonate Shampoo Solid-phase reaction (SPR-LC)... 

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