LC-MSMS

Casetta B, Tagliacozzi D, Shushan B. Development of a method for rapid quantitation of amino acids by liquid chromatography-tandem mass spectrometry (LC-MSMS) in plasma. Chn Chem Lab Med 2000 38 391-401. 

Xie, F.M. Bruntlett, C.S. Zhu, Y.X. Kissinger, C.B. Kissinger, P.T. Good Preclinical Bioanalytical Chemistry Requires Proper Sampling From Laboratory Animals Automation of Blood and Microdialysis Sampling Improves the Productivity of LC/MSMS, Anal. Sci. 19(4), 479 85 (2003). 

LC/NMR in various combinations with LC/UV-DAD, LC/MS, LC/MSMS, LC/IR, and/or LC/CD has been used in many applications related to the online identification of natural products. In this field, the challenge for hyphenated techniques is important since often the characterization of completely unknown molecules is required in very complex biological matrices. In this case, LC hyphenated techniques are used for the chemical evaluation of biologically active fractions or extracts and for dereplication purposes. As full structure assignment is often needed, all online spectroscopic data are taken into consideration. Most applications are performed in the stop-flow mode and extensive 2D NMR correlation experiments are measured. For unknown online determination the need for data is often mandatory. This type of information can be deduced from HSQC and HMBC indirect measurements and very recently it has been demonstrated that even direct measurements were possible in a crude plant extract. For this application the LC peak of interest was preconcentrated by trapping on SPE and the measurement was performed on a cryogenic flow... 

Casetta, B., Gravier, M., Belva, H., Tavet, Y., Cuvelier, 1. and Olichon, D., Quantitation by liquid chromatography-tandem mass spectrometry (LC-MSMS) of very long chain fatty acids (VLCFA) as a tool for the characterization of peroxisomal disorders. Poster D-130 at XIX International Congress of Clinical Chemistry/2005 AACC Annual Meeting. 

LC-MALDI MS- and MSMS runs 22067 MSMS spectra (mean 340/run)... 

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