Laser fluorometric detection

K. Sauda, T. Imasaka and N. Ishibashi, Determination of protein in human serum by high-performance liquid chromatography with semiconductor laser fluorometric detection, Anal. Chem. 58, 2649-2653 (1986). 

High-Performance Liquid Chromatography with Laser Fluorometric Detection... 

LIF has gradually become the most sensitive technique for analyte detection in narrow-bore capillaries e.g. in CE-LIF applications [10]). Also laser fluorometric detection for TLC was reported [122], LIF detection often relies on derivatisation of the target molecules, as most analytes do not show native fluorescence when being excited with commercial lasers. 

Swaile, D. F. and Sepaniak, M. J., Laser-based fluorometric detection schemes for the analysis of proteins by capillary zone electrophoresis, J. Liquid Ghromatogr., 14, 869, 1991. 

Paproski, R. E. Roy, K. I. Lucy, C. A. Selective fluorometric detection of polyamines using micellar electrokinetic chromatography with laser-induced fluorescence detection. J. Chromatogr, A 2002, 946, 265-273. 

Riboflavin can be assayed by chemical, en2ymatic, and microbiological methods. The most commonly used chemical method is fluorometry, which involves the measurement of intense yeUow-green fluorescence with a maximum at 565 nm in neutral aqueous solutions. The fluorometric deterrninations of flavins can be carried out by measuring the intensity of either the natural fluorescence of flavins or the fluorescence of lumiflavin formed by the irradiation of flavin in alkaline solution (68). The later development of a laser—fluorescence technique has extended the limits of detection for riboflavin by two orders of magnitude (69,70). 

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