Laser flows

Lyons AB, Samuel K, Sanderson A, Maddy AH. 1992. Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry. Cytometry 13 809-821. 

Schmid I, Krall WJ, Uittenbogaart CH, Braun J Giorgi JV. 1992. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13 204-208. 

The potential for multi-fluorochrome analysis has increased further with the availability of dyes excited by shorter wavelengths (such as Cascade blue) and longer wavelengths (such as Allophycocyamn and Texas red). With multiple laser flow cytometers, five cell-surface antigens (1) or more may be analyzed simultaneously. In principle, these methods are a natural extension of the methods outlined here, but require more sophisticated flow cytometers, and much more powerful computer facilities to explore the exponentially more complex data sets arising from such multiparameter analyses. 

Krishanswami and Rangaswami Ayyangar (1935) counted the haploid chromosome number to be 9 in E. indica, 18 in E. coracana, 18 in E. brevifolia, and 17 in . aegyptica, while Bisht and Mukai (2000) counted 36 chromosomes in . coracana, 18 in E. indica and E. tristachya, and 16 in E. multiflora. All these are annuals. For the perennials, E.floccifolia has 18, E. intermedia 18, and . jaegeri 20 chromosomes. Laser flow cytometry was... 

Mysore, K. S. and Baird, V. (1997). Nuclear DNA content in species of Eleusine (Gramineae) A critical re-evaluation using laser flow cytometry. Plant System. Evol. 207,1-11. 

N. L. Rapagnani and S. J. Davis, "Laser Induced Fluorescence Measurements in a Chemical Laser Flow Field," AIAA J. 

Dertinger, S. D., Torous, D. K., and Tometsko, K. R. (1996). Simple and reliable enumeration of micro-nucleated reticulocytes with a single-laser flow cytometer. Mutat Res 371, 283-292. 

To obtain IR spectra on a time scale of nanoseconds, the sample cell in conventional spectrometers is usually excited by an Nd YAG laser. Flow cells with a pathlength of at least 0.1 mm must be used for photoreactive samples and the pulse repetition frequency is then limited to 1 Hz. In step scan FTIR spectroscopy,211 the time evolution is collected at single points of the interferogram, which is then reconstructed point-by-point and subsequently transformed to time-resolved IR spectra. Alternatively, dispersive instruments equipped with a strong IR source can be used.212 The time resolution of both methods is about 50 ns. FTIR instruments provide a triggerable fast-scan mode to collect a complete spectrum within a few milliseconds.213... 

Bigos M, Baumgarth N, Jager GC, Herman OC, Nozaki T, Stovel RT, Parks DR, Herzenberg LA (1999) Nine color eleven parameter immunophenotyping using three laser flow cytometry. Cytometry 36 36-45... 

Intracellular dissolution of a large variety of inhaled inorganic particles not readily soluble in the pulmonary epithelial lining fluid constitutes an important long-term clearance mechanism of the lungs. Fluorescence microscope photometry and dual laser flow cytometry of intraphagolysosomal pH in... 

Dambaeva, S. V. Mazurov, D. V. Pinegin, B. V. Assessment of active oxygen form production in human peripheral blood using laser flow cytometry. Immunologiya 2001, 58-61. 

Figure 3. Flow cytometry analysis of apoptotic cells. HL-60 cells were treated with vehicle alone (A), 30 pM carnosic acid (B), 20 pM carnosol (C), or 40 pM ursolic acid (D) for 18 h. After 70% ethanol ftxation and propodium iodide (PI) staining, DNA content was analyzed by FACs laser flow cytometer.

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