Larva neuromuscular junction

F. 3 Comparison of the neuromuscular junction pattern and muscular fiber organization in control and clofibrate-treated larvae, using whole-mount acetylcholinesterase staining. 

Presynaptic kainate receptors control not only the release of glutamate and GABA.. In the striatum, for instance, GluR6 activation facilitated adenosine release, which then acted onto A2A receptors to inhibit evoked GABA release, whereas spontaneous GABA release was directly facilitated via kainate receptors (Chergui et al. 2000). At neuromuscular junctions of zebrafish larvae, activation of NR2A and kainate receptors increased spontaneous acetylcholine release (Todd et al. 2004). 

Figure 11.2. Neuromuscular junctions at muscles 12and 13 in the third-instar larva stained with presy-naptic antibody anti-horseradish peroxidase (HRP).

Figure 15.2. Drosophila larval neuromuscular junction system. A wandering third-instar larva is dissected open to reveal the ventral neuromusculature (see Figure 15.1). The peripheral nerve is severed and stimulated with a glass suction electrode. The muscle is recorded from in two-electrode voltage-clamp (TEVC) configuration. The postsynaptic excitatory junctional current (EJC) is recorded to assay synaptic transmission bottom inset). Top inset) Basis of synaptic transmission event being evoked by nerve stimulation and recorded via ion flux through muscle glutamate receptors.

Kidokoro Y. and Nishikawa K.-I. 1994. Miniature endplate currents at the newly formed neuromuscular junction in Drosophila embryos and larvae. Neurosci. Res. 19 143-154. 

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