Large dead volume union

It is usually a plot of peak intensity of the separated species over time, column The main component of the chromatographic separation. It is typically a tube containing the stationary-phase material that separates the species and an eluent that elutes the species off the column, counterions The mobile phase contains a large number of ions that have a charge opposite to that of the surface-bound ions. These are known as counterions, which establish equilibrium with the stationary phase, dead volume Usually refers to the volume of the mobile phase between the point of injection and the detector that is accessible to the sample species, minus the volume of mobile phase that is contained in any union or connecting tubing. 

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