Lactosylceramides

Fig. 3.—Mass Fragmentography of Methylated Neuraminic Acids. [Detection at m/e 274 A, disialosyl-lactosylceramide B, the disialosyl ganglioside isolated C, B after treatment with neuraminidase D, the fraction containing the trisialosyl ganglioside. Detection at m/e 330 E, the disialosyl ganglioside F, the trisialosyl fraction. The peaks identified are (1) the permethylated (terminal) N-acetylneuraminic acid, and (2) the 8-O-acetyl (8-O-substituted) derivative of the methylated N-acetylneuraminic acid. Conditions 1% of SE-30, at 240°. Reproduced, by permission, from Ref. 80.]...

LACTOSE SYNTHASE LACTOSE SYNTHASE Lactosylceramide a-2,6-N-sialyltransferase, SIALYLTRANSFERASE Lag phase in microtubule assembly, MICROTUBULE ASSEMBLY KINETICS Lag phase in polymerization,... 

Sialosyllactosylceramide (GM3) is one of the major gangliosicles found in visceral organs. However, in the central nervous system, the concentration of this ganglioside is so low that it can only be considered to be the precursor for various complex gangliosides. A sialosyl-transferase that catalyzes the transfer of sialic acid from CMP-NeuAc to lactosylceramide was first described in the embryonic chicken-brain by Basu107 and Kaufman and coworkers,108 and in the brain of... 

Other glycolipids from aquatic invertebrates are also characterized by a great variety of structures. Along with compounds known also to be present in vertebrates (gluco- and galacto-cerebrosides and lactosylceramide), new glycosphingolipids have been detected that differ from the glycosphin-... 

Prepare a 5 mg/ml liposome construction in 20 mM sodium phosphate, 0.15 M NaCl, pH 7.4, containing, on a molar ratio basis, a mixture of PC cholesterol PG other glycolipids of 8 10 1 2. The other glycolipids that can be incorporated include phosphatidyl inositol, lactosylceramide, galactose cerebroside, or various gangliosides. Other liposome compositions may be used, for example, recipes without cholesterol, as long as a periodate-oxidizable component... 

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