Lactophenol Blue

Preparation of the Lactophenol Blue reagent Solution A consists of 20 ml of lactic acid, 20 g of phenol, 40 ml of glycerine and 20 ml of distilled water. Solution B consists of 2 g of dye in 100 ml of distilled water. The reagent is prepared by mixing 50 ml of solution A and 10 ml of solution B. 

Pollen grains from FAA-preserved flowers on wild and greenhouse-grown plants were analyzed for cytoplasm stainability. Sterile or aborted pollen grains did not take up the cotton blue-lactophenol stain, and were also conspicuous by their shrunken size and shriveled form. 

All samples were examined with light microscopy. Sections were stained with either aqueous safranine or a solution of Chlorazol Sky-Blue (Imperial Chemical Industries, Ltd., U.K.) in lactophenol. Polarized light was occasionally employed. 

Epidermal strips were prepared [8], stained using cotton blue/ lactophenol for 5 minutes and mounted in lactophenol. Total haustorial counts for each epidermal strip were made and results expressed per unit leaf area. 

Lactophenol-cotton blue Prepare by mixing equal amounts of Components A and B described below ... 

Prepare lactophenol—cotton blue dye by mixing equal volumes of component A and component B. 

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