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Labeling of DNA

Polynucleotide kinase j Transfers terminal phosphate (y position) i from ATP to 5 -OH groups of DNA or RNA. P labeling of DNA or RNA. [Pg.400]

Forster, A.C., Mclnnes, J.L., Skingle, D.C., and Symons, R.H. (1985) Non-radioactive hybridization probes prepared by the chemical labeling of DNA and RNA with a novel reagent, photobiotin. Nucleic Acid Res. 13, 745-761. [Pg.1063]

DNA polymerase 1. For most applications DNA polymerase 1 from E. coli is used. The enzyme attaches to a short single-stranded region in a dsDNA molecule and then synthesizes a new strand of DNA, degrading the existing strand as it proceeds. When used in vitro this incubation is carried out at 12-15 °C to prevent more than one round of replication occurring. It is used for in vitro labelling of DNA by the nick translation method (described below). [Pg.460]

The use of NPs as labels of DNA molecules [20,21] opened a new alternative for the detection of hybridisation events. NPs labels offer a number of advantages for DNA detection platforms as well as for immunoassays. The NP-based DNA and immunoassays are easy to use, offer good sequence selectivity and sensitivity. Moreover, the NP labelling technology is compatible with chip techniques [22],... [Pg.943]

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3 -End labeling of DNA

Application of Field-Effect Transistors to Label-Free Electrical DNA Biosensor Arrays

Biotin Labeling of DNA

Covalent Labelling of Proteins and DNA

DNA labeling

DNA labels

DNA, labelled

Fluorescent Labeling of DNA

Labeled DNA

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