L-Dimethylaminonaphthalene-5-sulfonate

Beta-CD was functionalized at the positions 6-0- (70), 2-0- (71) and 3-0-(72) with l-dimethylaminonaphthalene-5-sulfonate [221], The absorption spectra of 70 and 71 were modified upon the addition of a guest. The absorption changes are larger for 70. At the same time, the fluorescence emission was quenched and the emission maximum was red-shifted by the addition of a guest. The fluorescence decay of 70 and 71 was fitted by two... 

Macromolecules may or may not fluoresce. Those that do are considered to contain intrinsic fluors. The common intrinsic fluors for proteins are tryptophan, tyrosine, and phenylalanine (the same three groups that absorb UV radiation). Macromolecules that have no intrinsic fluors can be made fluorescent by adding an extrinsic fluor to them. This is done by the process of chemical coupling or sample binding. The most common extrinsic fluors for proteins are l-aniline-8-naphthalene sulfonate, l-dimethylaminonaphthalene-5-sulfonate, dansyl chloride, 2-p-toluidyl-naphthalene-6-sulfonate, rhodamine, and fluorescein. The most common extrinsic fluor for nucleic acids are various acridienes (acridine orange, proflavin, acriflavin) and ethidium bromide. 

The following fluorescent probes were chosen as substrates N-phenyl-1-naphthylamine (PNA), 1-benzyl-1,4-dihydronicotinamide (BNAH), indole, indolyl-Sracetic acid, 8-anilinonaphthalene-l-sulfonate (ANS), 6-p-toluidinylnaphthalene-2-sulfonate (TNS), and l-dimethylaminonaphthalene-5-sulfonamidoethyltrimethylammonium (DASP). 

Koike, T., Abe, T., Takahashi, M., Ohtani, K., Kimura, E., and Shiro, M. Synthesis and characterisation of the zinc(II)-fluorophore, 5-dimethylaminonaphthalene-1 -sulfonic acid [2-(l,5,9-triazacyclododec-l-yl)ethyl]amide and its zinc(II) complex, Dalton Trans. (2002), 1764-1768. 

Once solvent corrections have been made, relative quantum efficiencies can be measured directly using fluorescence emission spectra. A standard for the determination of quantum yield, however, must also be run [9]. Typical standards whose quantum yields are known are quinine sulfate ( = 0.55), fluorescein ( = 0.85), and 5-dimethylaminonaphthalene-l-sulfonic acid, or DANS acid < > = 0.36 in 0.1 M NaHCOa [10]). 

Fig. 1. Dinitrophenyl (DNP) and 5-dimethylaminonaphthalene-l-sulfonic acid (DANS) peptides and amino acids...

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