Knockout serum replacer

Several investigators have evaluated that fibronectin-coated substrates maintain hESC pluripotency (Table 6.1) [51-58], whereas other researchers have reported unfavorable results when culturing hESCs on fibronectin-coated substrates [31,59]. Amit et al. cultured several hESC lines (1-6,1-3, and H-9) on bovine and human fibronectin-coated dishes (5 og/cm ) in knockout DMEM (KO-DMEM) supplemented with 15% serum replacement (SR). The fibronectin-specific integrin receptor aSpi was expressed in the undifferentiated hESCs (Figure 6.7) [52]. Under these conditions, the hESCs maintained pluripotency for more than six months, whereas the hESCs cultured on gelatin-coated dishes tended to differentiate [52]. Human fibronectin was found to be relatively more favorable for maintaining the pluripotency of the hESCs compared with bovine fibronectin [52]. The applied SR contained Albumax, which is aHpid-enriched bovine serum. Therefore, this work was not performed under xeno-free culture conditions. 

Cell quiescence under neither serum supplemented medium nor analogous knockout replacement Quiescence Quiescence Non-quiescence... 

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