Kinetic Properties of the Immobilized Zn II Complexes

For the kinetic assays a method had to be established which would allow determination of the kinetic parameters for BDNPP hydrolysis. In order to obtain initial 

For the complex MR-[Zn2(CH3L4)(CH3COO)2l no data above pH 8 were collected as the resin leached Zn(II) at alkaline pH [36]. This is in accordance to the findings in Chap. 7 where the free asymmetric Zn(II) complexes also showed this leaching of Zn(II) (hydroxide). As Fig. 8.14 demonstrates, the pH dependence of both complexes is similar to the free Zn(II) complexes (Chaps. 4 and 7). The pKa values were 7.61 0.71 and 6.41 0.31 for MR-[Zn2(CH3L4) 

34 mM were found (Fig. 8. 15a). Based on the estimated catalyst loading (based on the amount of ligand taken up by the resin) of 1.45 mmoFg the kcat is 11 6 x 10 s . These values are only an estimate as the errors were very large even though the experiment was conducted in triplicate. 

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