Kinetic Characteristics of Cyclic Enzyme Systems

The works presented in Tables 1.1 to 1.3 [76-86,109-122] deal only with theoretical aspects of the enzymic biochemical devices, and the biochemical devices were not carried into practice. Moreover, Okamoto [85] suggests using silicon technology instead of biomaterials for practical implementation of the device based on the cyclic enzyme system. 

This study is also based on the cyclic enzyme system, butits leading concept is to accomplish practical implementation of this system using biomaterials. In this respect, the analytical models developed here are related to several biochemical reactors in which enzymic reactions take place. This practical approach cannot be found in the models reviewed [76-86,109-122]. 

Intracellular communication often proceeds in a pulsatile, rhythmic manner [126]. Moreover, an increasing number of hormones are found to be secreted in a pulsatile manner, and the physiological efficiency of these signals appears to be closely related to their frequency [126]. Based on this understanding, a number of classes of drug therapies have been shown to require a periodic, pulsatile regimen of delivery for efficacy or optimization [131], and several delivery strategies have been proposed to respond to this need [127-131]. 

Glucose-6-phosphate + NADP gluconate-6-phosphate + NADPH 

93 mM glycine-NaOH buffer pH 9.1, also containing 9.3 mM MgCp and 0.93 mM EDTA 

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