Isozyme banding patterns

Figure 2. Isozymer banding pattern in PAG of Protogonyaulax tamarensis morphotypes stained for malic enzyme (ME/NADP-dependent malate dehydrogenase).400-406 English Bay isolates ( indicates clonal). 255 Lummi Island (Washington State).

Figure 3. Isozyme banding patterns of Protogonyaulax morphotypes stained for glutamate dehydrogenase (GDH/NAD-dependent).

Figure 2. Densitometer tracings illustrating the isozyme migration patterns of five EAP phenotypes during electrophoresis for 4 hr at ()°C. The s and s storage bands can also be seen.

Figure 6. The effect of neuraminidase on the EAP phenotype patterns obtained during electrophoresis for 4 hr at 0°C. Samples 1 4 contained no neuraminidase. Samples 5-9 contained 10 units of neuraminidase activity per ml. All samples were incubated at 25°C. The photograph taken at 114 hr was exposed for 6 min to enhance EAP activity observed in samples 1 4. A 2-min exposure was used for photographs taken at the other times. The only EAP activity observed after incubation with neuraminidase for 114 hr was the c isozyme band of sample 5 (EAP phenotype AC). The c isozyme is the most stable of the four EAP isozymes (5).

CB, AC, and C (the rare type C was not observed in this study). Electrophoresis for 4 hours at 0 C consistently gave sharper EAP banding patterns than electrophoresis for 15 hours at 5 C. The a isozyme of types AB and AC was well defined during 4 hour electrophoresis and made the typing of EAP phenotype AB reliable and simple. 

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