Isotopic tracers tricarboxylic acid cycle

BOX 17-C USE OF ISOTOPIC TRACERS IN STUDY OF THE TRICARBOXYLIC ACID CYCLE... 

The newer development began in 1942 when Lynen - reported experiments which in his view supported the occurrence of the tricarboxylic acid cycle in yeast cells, but the evidence was not generally accepted as conclusive because it fell short in satisfying the quantitative requirements. Since 1949, however, new observations, especially those based on the use of isotopic tracers, have lent support to the view that the cycle represents the main terminal pathway of oxidation in some microorganisms. Microorganisms, however, show a greater variety of metabolic properties than do animals, and there is much evidence indicating the occurrence of other intermediary pathways. 

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