Isotope effects methane monooxygenase

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Some indication of active-site acidic functionality in P450 dioxygen scission can be revealed by the comparative evaluation of kinetic parameters in protonated and deuterated waters. The use of kinetic solvent isotope effects (KSIEs) has been valuable in the determination of potential proton-linked dioxygen activating enzymes such as P450 -, methane monooxygenase (MMO), ... 

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